High-risk human papillomavirus (HPV) infection is involved in the development of anogenital cancers. To understand the immunological consequences of chronic HPV infection in pre-cancer and cancer, we used RNA-sequencing to examine transcriptomic changes that is associated with immune cell infiltration, regulatory cytokine expression and immunosuppression. This is performed using a transgenic mouse model expressing HPV E7 oncoprotein in keratinocytes, modelling skin epithelial pre-malignancy (K14E7). We observed up-regulation of transcripts encoding chemokine ligands and receptors (Cxcl9, Cxcl10, Cxcl4, Cxcr3, Ccr5) and immune checkpoint molecules (Ctla4, Pdcd1, Cd274, Pdcd1lg2) in K14E7 skin and these gene changes were validated at the protein level and can also be found to enriched in publically available microarray datasets of human CIN3 lesions. To separate the effects found in an admixture of cells from a bulk sample, we performed single-cell RNA sequencing on keratinocytes derived from E7-expressing and normal control murine skin. Interestingly, we discovered that Il33 mRNA expression is lost in K14E7 keratinocytes, predicted to be during early stages of keratinocyte differentiation/stage along a pseudo-time scale, suggesting that IL33 suppression may be a mechanism by which E7-expressing cells avoid immune surveillance and interfere with effector T cell function. Taken together, the data is supporting the overall hypothesis that HPV-associated hyperproliferative epithelium induces stress signals and expresses chemokine signals to drive immune cell infiltrate into this environment but at the same time, preferentially suppress expression of epithelial damage signals.