Introduction:
Systemic inflammation is associated with endothelial injury. Endothelial cells release biomarkers which can be used as diagnostic tools of inflammatory response. There is limited information regarding endothelial dysfunction in newborns. Aim of our study was to explore microvesicles and biomarkers of endothelial injury in critically ill newborns.
Methods:
Biomarkers were measured in newborns on extracorporeal membrane oxygenation (ECMO) and compared to healthy term newborns. The total microvesicle (MV) count and number endothelial MV was determined by flow cytometry (BD FACS CantoII). The plasma concentration of MV was measured using annexin-V labeling, endothelial origin determined using CD105, CD31, CD309, MadCAM antigens. Serum soluble biomarkers were measured using multiplex immunoassays based Luminex®xMAP multi-analyte platform.
Results:
13 newborns on ECMO and 13 healthy term newborns were included. There were no significant differences in gestational age, birthweight and gender between groups. The concentration of markers shows Table1.
Table 1.
|
Inflammatory and endothelial markers |
ECMO group |
Term group
|
|
Procalcitonin [ng/ml] |
16.22(3.749)** |
0.68(0.26)** |
|
IL-1beta [pg/ml] |
9.98(2.53)** |
1.67(0.31)** |
|
IL-6 [pg/ml] |
2043.00(1330)** |
7.33(0.82)** |
|
Endocan [ng/ml] |
2789(271)** |
1496(128)** |
|
Angiopoietin-2 [ng/ml] |
21.44(6.26)** |
6.93(0.75)** |
|
VEGF [pg/ml] |
4.19(1.17)** |
88.5(21.19)** |
|
Endothelin-1 [pg/ml] |
6.63(1.18) |
5.49(1.90) |
|
MV total (Annexin-V), [MV/l] |
1672(464)** |
354(94)** |
|
MV (Annexin-V/CD31), [MV/l] |
346(117) |
115(49) |
|
MV (Annexin-V/MadCAM), [MV/l] |
108(38)* |
21(8)* |
|
MV (Annexin-V/ VEGFR2), [MV/l] |
86(34) |
74(35) |
Data presented as mean (SEM). **P < 0.01, *P < 0.05
Conclusion:
Markers of inflammation and concentration of MV were significantly increased in ECMO group. Endocan and angiopoietin-2 were increased in ECMO group showing potential endothelial impairment. We did not detect significant differences in endothelial MV between the groups, with the exception of mucosal endothelium marker MadCAM.
Supported by project 16-27800A, Endothelial injury in newborns, Ministry of Health, Czech Republic, Medical and Research Program, Ministry of Health, Czech Republic, MHCZ-DRO Thomayer Hospital-TN0064190.